Small Animal Biologist

Hope you are doing great! Please go through the below JD and let me know if you are interested in this role, Feel free to reach me in case you have any query, You can call me or email me at WORK LOCATION Job title : Small Animal Biologist Job location : Bethesda, Maryland 20814 Job type : Contract (onsite) WORK HOURS Weekly Hours FT: 30-40 hours per week Number of Hours 40 SHIFT SCHEDULE On-Call or Rotational Shifts No Teleworking Eligible Yes Nights No Holidays No Fixed Schedule No Remote No Onsite Yes POSITION INFORMATION Overall Position Summary and Objectives This position will independently provide support services to satisfy the overall operational objectives of the NCI/CCR. Minimum Education Bachelor's ADDITIONAL QUALIFICATIONS Certifications & Licenses

• Bachelor's degree in Biology, Life Sciences or a related discipline
• Min 5 years laboratory exp in molecular biology, cellular biology, microbiology or a related
• Min 3 years laboratory experience in mouse handling
• Bachelors degree in Biology, Life Sciences or related discipline

• MS Office Suite
• Filemaker Pro (preferred)

• Miscellaneous Biology
• Molecular Biology
• Animal Sciences

• Work products and documents related to performing basic and translational research to test hypotheses developed by MyPART leadership; process samples collected by the MyPART network using molecular biology techniques. - Ad-Hoc

• Work products and documents related to working closely with core facilities and outside vendors to maintain chain of custody of biospecimens and identify any areas for improvement of standard operating procedures; perform molecular, cellular, and microbiology techniques in accordance with Biosafety Level 2 guidelines. - Ad-Hoc

• Work products and documents related to performing techniques of molecular biology, including PCR, quantitative RT-PCR, TaqMan, gene cloning, vector construction and cell transfection and transduction; perform cell culture, FACS and ELISA. - Ad-Hoc

• Work products and documents related to updating and maintaining laboratory databases and notebooks and standard operating procedures; collect, analyze and interpret data; prepare reports and summaries based on findings and results; attend and participate in seminars and meetings. - Ad-Hoc

• Clean Equipment - Bi-Weekly

• Meet with lab members to present updates - Weekly

• Work products and documents related to managing health and welfare of mice in animal colony, and to completion of tasks such as culling mice, setting up breeders, measuring tumor growth, and administering experiemental therapies. - Weekly

• Perform behavioral assays in association with the primary research project.

• Coordinate husbandry, animal care and enrichment activities to maintain a clean, safe and regulatory compliant animal research program.

• Work with staff to maintain, breed, genotype and phenotype transgenic animal colony; maintain records of animal colony.

• Independently perform genotyping of animals.

• Set up animals to be available in numbers and at the time required.

• Update animal protocol through the submission of amendments and submission of new animal protocols to ensure necessary animal work for experiments.

• Provide technical expertise in breeding colony management.

• Maintain existing database to record all animals in breeding colony, tracking lineage, genotype, birth, death, and experimental use of each animal

• Manage and breed animal colonies; genotype animals and report genotyping results to staff.

• Communicate and collaborate with investigators regarding colony and support duties.

• Maintain an extensive database repository that tracks the life and health cycles of the entire transgenic colony, including animal breeding, health, and housing areas.

• Inspect all animals each day for viability and health status.

• Handle animals for hormone injection, mating, timed mating, and other experimental procedures.

• Work with staff to maintain, breed, genotype and phenotype transgenic animal colony; maintain records of animal colony.

• Handle, dissect and euthanize animals.

• Work with staff on maintaining animal colonies, including setting up mating pairs and checking plugs, genotyping animals by tail biopsy, injecting animals with hormones or other compounds; inject cells into immunocompromised animals; monitor and analyze tumor growth.

• Maintain animal colony, including general husbandry, breeding, plug checks, weaning, tagging animals and obtaining tail or ear snips for genotyping, and euthanasia.

• Handle, dissect and euthanize animals.

• Perform genomic DNA preparation, genotyping and other analyses of animal tissues using DNA extraction and PCR.

• Perform tissue and cell culture; use sterile technique.

• Carry out molecular biological experiments, including extracting genomic DNA, performing PCR and gel electrophoresis, cloning DNA fragments and preparing plasmid DNA, generating high quality RNA using in vitro transcription.

• Perform standard molecular biology techniques, including, but not limited to, cloning, in situ hybridization and qPCR.

• Maintain cell lines and perform basic cell culture assays, including, but not limited to, transfection and immunocytochemistry.

• Perform apoptosis assays, qtr.-PCR, Western blotting, immunohistochemistry and molecular cloning techniques.

• Work closely with core facilities and outside vendors to maintain chain of custody of biospecimens and identify any areas for improvement of standard operating procedures.

• Perform molecular, cellular, and microbiology techniques in accordance with Biosafety Level 2 guidelines and be proficient in mouse handling.

• Perform administrative duties including ordering, managing budgets under the direction of the MyPART Leadership, maintain written documentation of standard operating procedures and research findings.

• Perform techniques of molecular biology, including PCR, quantitative RT-PCR, TaqMan, gene cloning, vector construction and cell transfection and transduction.

• Perform FACS and ELISA.

• Update and maintain laboratory databases and notebooks.

• Attend and participate in seminars and meetings.

• Update and maintain laboratory notebooks and databases; collect, analyze and interpret data.

• Perform quantitative analysis; data analysis, figure preparation, and image acquisition; maintain complete and detailed records of all experiments.

• Collect, organize and record experimental data for publication and future reference by others.

• Collect, review, research, and analyze large datasets; perform experiments, utilizing various statistical applications and methods; and interpret data, assemble data into figures, and enter data into a database.

• Analyze and interpret scientific data from animal cell lines.

• Collate, edit and archive electronic and paper laboratory files, notebooks and databases.

• Analyze and catalog imaging data from molecular biology and in vitro tissue culture assays.

• Edit and write research papers and protocols.

• Prepare abstracts and present posters.

• Participate and collaborate in the preparation of scientific publications for peer reviewed journals based on the results.

• Document information on procedures, modifications and methods for input into final publication and presentations of findings at meetings.

• Work with staff on the modification of laboratory protocols and experiments based on literature findings.

• Develop, validate, perform and interpret a variety of complex experimental techniques and procedures; work with staff to develop procedures addressing new animal study needs, logistics, modifications to standard operating procedures, and/or existing guidelines to resolve technical problems.

• Collaborate with team members to determine, evaluate and discuss the progress of projects; point out problems and suggest solutions to problems or augmentation of experimental approaches.

• Locate and review the literature pertinent to projects to identify the most applicable protocols and methods; perform experiments in accordance with protocols.